How to clean a biological safety cabinet lafw hood.
Cleaning procedure for biological safety cabinet.
Biological safety cabinet sop bsc 01.
Cleaning and disinfection 1.
In addition periodic and thorough decontamination routines including disinfecting all removable parts and surfaces is recommended by the manufacturer and is a good laboratory practice to reduce wear on the equipment and provide greater safety to you your samples and the environment.
If the biological safety cabinet is turned off between aseptic processes for routine maintenance or any other reason it should be operated long enough to allow complete purging of room air from the critical area for at least 30 minutes then cleaned and disinfected before use.
5 0 procedure 5 1 general cleaning procedure 5 1 1 ensure that the power supply of the biosafety cabinet is switched off.
Aseptic technique and daily decontamination will eliminate the majority of contaminants.
5 1 2 dedust the biosafety cabinet daily externally with a clean dry cloth.
Spray the surfaces of all items placed into the cabinet with 70 ethanol to minimize the introduction of contaminants into the bsc.
10 easy steps to cleaning a contaminated surface in the biosafety cabinet.
Cleaning a biosafety cabinet or bsc is an important function in terms of both containment and sterility.
Put the waste from the spill into a biohazard bag inside the cabinet.
Once the cabinet has been operational for 15 minutes disinfect all interior surfaces of the cabinet thoroughly by spraying with 70 ethanol.
Allow to air dry.
The interior surfaces should also be wiped with an appropriate disinfectant that would kill any microorganisms that could be found in the cabinet.
Remember to wear appropriate ppe and leave the biosafety cabinet on during spill cleanup.
This may take more or less time depending on the disinfectant and the microbiological agent used.
μ microns in diameter with higher efficiency for both larger and.
The biohazard bag should never be kept outside the cabinet.
Afterward wipe the surface with a clean dry cloth to remove the moisture.
How to clean a biological safety cabinet lafw hood.
When work is completed all equipment and supplies from the bsc should be decontaminated and removed from the cabinet.
Hepa stands for high efficiency particulate air.
Hepa filters are defined as air cleaning devices that have a proven minimum removal efficiency of 99 97 of particles in the air equal to 0 3.
Typically surface decontamination is adequate however there are instances such as extensive repair inside the cabinet envelope or hepa filter replacement that will require gas decontamination.
The biological safety officer will determine the type of decontamination necessary.
To begin apply disinfect to a towel and decontaminate and remove items from the area of the spill.
